IL-7R signaling activates widespread V_H and D_H gene usage to drive antibody diversity in bone marrow B cells.

ABSTRACT: Generation of the primary antibody repertoire requires V(D)J recombination of hundreds of gene segments in the immunoglobulin heavy chain (Igh) locus. The role of interleukin-7 receptor (IL-7R) signaling in Igh recombination has been difficult to partition from its role in B cell survival and proliferation. With a detailed description of the Igh repertoire in murine IL-7Rα^-/- bone marrow B cells, we demonstrate that IL-7R signaling profoundly influences V_H gene selection during V_H-to-DJ_H recombination. We find skewing toward 3' V_H genes during de novo V_H-to-DJ_H recombination more severe than the fetal liver (FL) repertoire and uncover a role for IL-7R signaling in D_H-to-J_H recombination. Tran

SUBMITTER: Baizan-Edge A 

PROVIDER: S-EPMC8293627 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature