CRISPR/Cas9-Mediated Whole Genomic Wide Knockout Screening Identifies Specific Genes Associated With PM_2.5-Induced Mineral Absorption in Liver Toxicity.

ABSTRACT: PM_2.5, also known as fine particles, refers to particulate matter with a dynamic diameter of ≦2.5 μm in air pollutants, that carries metals (Zn, Co, Cd) which can pass through the alveolar epithelium and enter the circulatory system and tissues. PM_2.5 can cause serious health problems, such as non-alcoholic fatty liver and hepatocellular carcinoma, although the underlying mechanisms of its toxic effect are poorly understood. Here, we exposed L02 cells to PM_2.5 and performed a pooled genome-wide clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) to assess loss of function and identify new potential PM_2.5targets. Enrichr and KEGG pathway analyses were performed to identify candidate genes associated

SUBMITTER: Peng J 

PROVIDER: S-EPMC8293916 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature