Polymorphism, expression and structure analysis of key genes in the ovarian steroidogenesis pathway in sheep (Ovis aries).

ABSTRACT:

Background

Litter size is an important factor that significantly affects the development of the sheep industry. Our previous TMT proteomics analysis found that three key proteins in the ovarian steroidogenesis pathway, STAR, HSD3B1, and CYP11A1, may affect the litter size trait of Small Tail Han sheep.

Objective

The purpose of this study was to better understand the relationship between polymorphisms of these three genes and litter size.

Material and method

Sequenom MassARRAY detected genetic variance of the three genes in 768 sheep. Real-time qPCR of the three genes was used to compare their expression in monotocous and polytocous sheep in relevant tissues. Finally, bioinformatics analysis predicted the protein sequences of the different SNP variants.

Result

A

SUBMITTER: Hu WP 

PROVIDER: S-EPMC8294399 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature