Gene expression changes in male and female rhesus macaque 60 days after irradiation.

ABSTRACT:

Purpose

Transcriptome changes can be expected in survivors after lethal irradiation. We aimed to characterize these in males and females and after different cytokine treatments 60 days after irradiation.

Material and methods

Male and female rhesus macaques (n = 142) received a whole-body exposure with 700 cGy, from which 60 animals survived. Peripheral whole blood was drawn pre-exposure and before sacrificing the surviving animals after 60 days.

Results

We evaluated gene expression in a three-phase study design. Phase I was a whole-genome screening (NGS) for mRNAs using five pre- and post-exposure RNA samples from both sexes (n = 20). Differential gene expression (DGE) was calculated between samples of survivors and pre-exposure samples (reference), separately for mal

SUBMITTER: Majewski M 

PROVIDER: S-EPMC8294544 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature