Live imaging and biophysical modeling support a button-based mechanism of somatic homolog pairing in Drosophila.

ABSTRACT: Three-dimensional eukaryotic genome organization provides the structural basis for gene regulation. In Drosophila melanogaster, genome folding is characterized by somatic homolog pairing, where homologous chromosomes are intimately paired from end to end; however, how homologs identify one another and pair has remained mysterious. Recently, this process has been proposed to be driven by specifically interacting 'buttons' encoded along chromosomes. Here, we turned this hypothesis into a quantitative biophysical model to demonstrate that a button-based mechanism can lead to chromosome-wide pairing. We tested our model using live-imaging measurements of chromosomal loci tagged with the MS2 and PP7 nascent RNA labeling systems. We show solid agreement between model predictions and exper

SUBMITTER: Child MB 

PROVIDER: S-EPMC8294847 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature