Characterization and visualization of murine coagulation factor VIII-producing cells in vivo.

ABSTRACT: Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31^high, CD146^high, and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1)⁺. EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin^- and C-type lectin-like receptor-2 (CLEC-2)⁺. In embry

SUBMITTER: Hayakawa M 

PROVIDER: S-EPMC8295325 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature