Identification of protein interactions of grapevine fanleaf virus RNA-dependent RNA polymerase during infection of Nicotiana benthamiana by affinity purification and tandem mass spectrometry.

ABSTRACT: The RNA-dependent RNA polymerase (1E^Pol) is involved in replication of grapevine fanleaf virus (GFLV, Nepovirus, Secoviridae) and causes vein clearing symptoms in Nicotiana benthamiana. Information on protein 1E^Pol interaction with other viral and host proteins is scarce. To study protein 1E^Pol biology, three GFLV infectious clones, i.e. GHu (a symptomatic wild-type strain), GHu-1E_K802G (an asymptomatic GHu mutant) and F13 (an asymptomatic wild-type strain), were engineered with protein 1E^Pol fused to a V5 epitope tag at the C-terminus. Following Agrobacterium tumefaciens-mediated delivery of GFLV clones in N. benthamiana and protein extraction at seven dpi, when optimal 1E^Pol:V5 accumulati

SUBMITTER: Osterbaan LJ 

PROVIDER: S-EPMC8295916 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature