Ethanol affects fibroblast behavior differentially at low and high doses: A comprehensive, dose-response evaluation.

ABSTRACT: This study aims to develop a comprehensive understanding of effects of low and high doses of ethanol on cellular biochemistry and morphology. Here, fibroblast cells are exposed to ethanol of varied concentrations [0.005-10 % (v/v)] to investigate cellular activity, cytoskeletal organization, cellular stiffness, mitochondrial structure, and real-time behavior. Our results indicate a sharp difference in cellular behavior above and below 1 % ethanol concentration. A two-fold increase in MTT activity at low doses is observed, whereas at high doses it decreases. This increased activity at low doses does not involve cell proliferation changes or mitochondrial impairment, as seen at higher doses. Moreover, the study identifies different types of mitochondrial structure impairment at high doses. M

SUBMITTER: Kar N 

PROVIDER: S-EPMC8296147 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature