Characterization and Transcriptome Analysis of a Long-Chain n-Alkane-Degrading Strain Acinetobacter pittii SW-1.

ABSTRACT: Strain sw-1, isolated from 7619-m seawater of the Mariana Trench, was identified as Acinetobacter pittii by 16S rRNA gene and whole-genome sequencing. A. pittii sw-1 was able to efficiently utilize long-chain n-alkanes (C₁₈-C₃₆), but not short- and medium-chain n-alkanes (C₈-C₁₆). The degradation rate of C₂₀ was 91.25%, followed by C₁₈, C₂₂, C₂₄, C₃₂, and C₃₆ with the degradation rates of 89.30%, 84.03%, 80.29%, 30.29%, and 13.37%, respectively. To investigate the degradation mechanisms of n-alkanes for this strain, the genome and the transcriptome analyses were performed. Four key alkane hydroxylase genes (alkB, almA, ladA1,

SUBMITTER: Kong W 

PROVIDER: S-EPMC8296198 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature