Characterizing Multidevice Capillary Vibrating Sharp-Edge Spray Ionization for In-Droplet Hydrogen/Deuterium Exchange to Enhance Compound Identification.

ABSTRACT: Multidevice capillary vibrating sharp-edge spray ionization (cVSSI) source parameters have been examined to determine their effects on conducting in-droplet hydrogen/deuterium exchange (HDX) experiments. Control experiments using select compounds indicate that the observed differences in mass spectral isotopic distributions obtained upon initiation of HDX result primarily from solution-phase reactions as opposed to gas-phase exchange. Preliminary studies have determined that robust HDX can only be achieved with the application of same-polarity voltage to both the analyte and the deuterium oxide reagent (D₂O) cVSSI devices. Additionally, a similar HDX reactivity dependence on the voltage applied to the D₂O device for various analytes is observed. Analyte and rea

SUBMITTER: DeBastiani A 

PROVIDER: S-EPMC8296548 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature