RNA sequencing identifies global transcriptional changes in peripheral CD4⁺ cells during active oesophagitis and following epicutaneous immunotherapy in eosinophilic oesophagitis.

ABSTRACT:

Objective

There are no disease-modifying therapies for the treatment of eosinophilic oesophagitis (EoE), which is driven by non-IgE-mediated allergic inflammation. A recent clinical trial of milk epicutaneous immunotherapy (EPIT) has shown initial promise, with 47% of treated EoE patients tolerating milk without recurrence of disease. Mechanisms of EPIT in EoE have not been studied in humans. Here, we identify transcriptional changes in the peripheral CD4⁺ T-cell compartment during active EoE and following EPIT.

Methods

RNA isolation, sequencing and integrative data analysis were performed on peripheral CD4⁺ T cells isolated from 15 of 20 patients enrolled in a clinical trial of EPIT for EoE. Gene expression changes in peripheral CD4⁺ T cells

SUBMITTER: Ruffner MA 

PROVIDER: S-EPMC8296633 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature