Microglia Phenotypes Following the Induction of Alcohol Dependence in Adolescent Rats.

ABSTRACT:

Background

Activation of the innate immune system may play a role in the development of alcohol use disorders (AUDs), which often originate with adolescent alcohol abuse. A key player in the innate immune system is microglia, the activation of which occurs along a spectrum from proinflammatory, or M1-like, to anti-inflammatory, or M2-like, phenotypes.

Methods

Adolescent, male rats were gavaged with ethanol (EtOH) or isocaloric control diet every 8 hours for 4 days and then sacrificed at 0, 2, 7, and 14 days later. Microglia were isolated from the entorhinal cortex and hippocampus by Percoll gradient centrifugation, labeled with surface antigens for activation, and analyzed by flow cytometry. Polarization states of microglia, defined as CD11b⁺ CD45^low ce

SUBMITTER: Peng H 

PROVIDER: S-EPMC8296648 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature