Construction of DNA Tools for Hyperexpression in <i>Marchantia</i> Chloroplasts.

ABSTRACT: Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort Marchantia polymorpha is a simple model plant that allows rapid transformation studies; however, its potential for protein hyperexpression has not been fully exploited. This is partially due to the fact that chloroplast post-transcriptional regulation is poorly characterized in this plant. We have mapped patterns of transcription in Marchantia chloroplasts. Furthermore, we have obtained and compared sequences from 51 bryophyte species and identified putative sites f

SUBMITTER: Frangedakis E 

PROVIDER: S-EPMC8296666 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature