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Genome Sequence of an Okra Leaf Curl Virus from Egypt.

ABSTRACT: A complete Okra leaf curl virus DNA-A was sequenced from okra in Egypt. Here, we report the complete genome sequence of this monopartite virus, comprising 2,764 bp and encoding 6 open reading frames (ORFs) with a GC content of 44.6% and 88.3% similarity to a virus reported earlier from Cameroon.

SUBMITTER: Magdy M

PROVIDER: S-EPMC8297448 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Genome Sequence of an <i>Okra Leaf Curl Virus</i> from Egypt.

Magdy Mahmoud M Appiah Andrew S AS Rizk Samah M SM Elhifnawi Hagar T HT Karikari Benjamin B Ahiakpa John K JK El-Dougdoug Noha K NK

Microbiology resource announcements 20210722 29

A complete <i>Okra leaf curl virus</i> DNA-A was sequenced from okra in Egypt. Here, we report the complete genome sequence of this monopartite virus, comprising 2,764 bp and encoding 6 open reading frames (ORFs) with a GC content of 44.6% and 88.3% similarity to a virus reported earlier from Cameroon. ...[more]

PMID: 34292069

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Project description:BackgroundBegomoviruses are whitefly-transmitted geminiviruses with genomes that consist of either two components (known as DNA A and DNA B) or a single component (homologous to the DNA A component of bipartite begomoviruses). Monopartite begomoviruses are often associated with a symptom-modulating DNA satellite (collectively known as betasatellites). Both bipartite and monopartite begomoviruses with associated satellites have previously been identified in chillies showing leaf curl symptoms in Pakistan.ResultsA chilli plant (Capsicum annum) with chilli leaf curl disease symptoms was found to contain a begomovirus, a betasatellite and the DNA B component of Tomato leaf curl New Delhi virus (ToLCNDV). The begomovirus consisted of 2747 nucleotides and had the highest sequence identity (99%) with Pepper leaf curl Lahore virus (PepLCLV-[PK: Lah:04], acc. no. AM404179). Agrobacterium-mediated inoculation of the clone to Nicotiana benthamiana, induced very mild symptoms and low levels of viral DNA, detected in systemically infected leaves by PCR. No symptoms were induced in Nicotiana tabacum or chillies either in the presence or absence of a betasatellite. However, inoculation of PepLCLV with the DNA B component of ToLCNDV induced leaf curl symptoms in N. benthamiana, N. tabacum and chillies and viral DNA accumulated to higher levels in comparison to plants infected with just PepLCLV.ConclusionsBased on our previous efforts aimed at understanding of diversity of begomoviruses associated with chillies, we propose that PepLCLV was recently mobilized into chillies upon its interaction with DNA B of ToLCNDV. Interestingly, the putative rep-binding iterons found on PepLCLV (GGGGAC) differ at two base positions from those of ToLCNDV (GGTGTC). This is the first experimental demonstration of the infectivity for a bipartite begomovirus causing chilli leaf curl disease in chillies from Pakistan and suggests that component capture is contributing to the emerging complexity of begomovirus diseases in the region.

Dataset Information

Genome Sequence of an Okra Leaf Curl Virus from Egypt.

Publications

Genome Sequence of an <i>Okra Leaf Curl Virus</i> from Egypt.

Similar Datasets

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