Desiccation Induced Conjunctival Monocyte Recruitment and Activation - Implications for Keratoconjunctivitis.

ABSTRACT:

Background

Lacrimal gland secretory dysfunction in Sjögren syndrome (SS) causes ocular surface desiccation that is associated with increased cytokine expression and number of antigen-presenting cells (APCs) in the conjunctiva. This study evaluated the hypothesis that desiccating stress (DS) alters the percentage and gene expression of myeloid cell populations in the conjunctiva.

Methods

DS was induced by pharmacologic suppression of tear secretion and exposure to drafty low humidity environment. Bone marrow chimeras and adoptive transfer of CD45.1⁺ bone marrow cells to CD45.2⁺ C-C chemokine receptor 2 knockout (CCR2^-/-) mice were used to track DS-induced myeloid cell recruitment to the conjunctiva. Flow cytometry evaluated myeloid cell popula

SUBMITTER: Alam J 

PROVIDER: S-EPMC8297564 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature