Dataset Information

3D Particle Free Printing of Biocompatible Conductive Hydrogel Platforms for Neuron Growth and Electrophysiological Recording.

ABSTRACT: Electrically conductive 3D periodic microscaffolds are fabricated using a particle-free direct ink writing approach for use as neuronal growth and electrophysiological recording platforms. A poly (2-hydroxyethyl methacrylate) (pHEMA)/pyrrole ink, followed by chemical in situ polymerization of pyrrole, enables hydrogel printing through nozzles as small as 1 μm. These conductive hydrogels can pattern complex 2D and 3D structures and have good biocompatibility with test cell cultures (~94.5% viability after 7 days). Hydrogel arrays promote extensive neurite outgrowth of cultured Aplysia californica pedal ganglion neurons. This platform allows extracellular electrophysiological recording of steady-state and stimulated electrical neuronal activities. In summation, this 3D conductive ink printing process enables preparation of biocompatible and micron-sized structures to create customized in vitro electrophysiological recording platforms.

SUBMITTER: Wang C

PROVIDER: S-EPMC8297588 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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3D Particle Free Printing of Biocompatible Conductive Hydrogel Platforms for Neuron Growth and Electrophysiological Recording.

Wang Chen C Rubakhin Stanislav S SS Enright Michael J MJ Sweedler Jonathan V JV Nuzzo Ralph G RG

Advanced functional materials 20210127 14

Electrically conductive 3D periodic microscaffolds are fabricated using a particle-free direct ink writing approach for use as neuronal growth and electrophysiological recording platforms. A poly (2-hydroxyethyl methacrylate) (pHEMA)/pyrrole ink, followed by chemical <i>in situ</i> polymerization of pyrrole, enables hydrogel printing through nozzles as small as 1 μm. These conductive hydrogels can pattern complex 2D and 3D structures and have good biocompatibility with test cell cultures (~94.5% ...[more]

PMID: 34305503

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Project description:BackgroundThree-dimensional (3D) printing technology has been utilized to create patient-specific (PS) replicas as visual aids for surgical planning.1-4 However, they cannot recreate the operative experience due to a lack of realistic tissue characteristics.ObjectivesDevelop anatomically accurate, realistic, PS partial nephrectomy platforms suitable for pre-operative surgical rehearsals using 3D-printing and hydrogel casting.MaterialPatient CT scans were segmented into a computer-aided design (CAD) file and used to create injection casts. Kidney and tumor casts along with hollow vascular and urinary structures were 3D-printed. The hilar structures and tumor were registered into the kidney cast, injected with poly-vinyl alcohol (PVA) hydrogel, and processed to create the kidney phantom. Mechanical and functional testing protocols were completed to confirm that the properties of PVA matched the live tissue.5 Anatomical accuracy was confirmed by CT scanning the phantom and creating another CAD, which was compared to the original patient CAD. Full-procedural PS rehearsals were completed 24-48 hours prior to their respective live surgeries. Clinically relevant metrics (warm ischemia time, estimated blood loss, and positive surgical margins) from each rehearsal and live case were compared using a Wilcoxon-rank sum test.ResultsThe 7%-3freeze/thaw PVA best recreated the mechanical and functional properties of porcine kidneys, while anatomical verification showed ≤1 mm deviation of the kidney and tumor from the patient anatomy and ≤3 mm for the hilar structures. PS rehearsal platforms were fabricated using these methods for 8 patients (average tumor size 5.92 cm and nephrometry score 9.8). A positive correlation was found for warm ischemia time and estimated blood loss between rehearsals and live surgeries.ConclusionThis reproducible method shows high anatomical accuracy, realistic tissue properties, and translational effects between rehearsals and live surgery. To determine the effects on patient outcomes, future studies will compare the impact of completing a pre-operative rehearsal vs standard surgical preparation.

Dataset Information

3D Particle Free Printing of Biocompatible Conductive Hydrogel Platforms for Neuron Growth and Electrophysiological Recording.

Publications

3D Particle Free Printing of Biocompatible Conductive Hydrogel Platforms for Neuron Growth and Electrophysiological Recording.

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