FFPE samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas.

ABSTRACT: During surgical procedures for gliomas, tissue material obtained from cavitational ultrasonic surgical aspirators (CUSAs) is generally discarded but can actually exceed the amount and quality of certain tumour core resections (TCRs). Despite reports indicating the suitability of CUSA-derived material for diagnosis and research, its use is still marginal. We extended these conclusions to formalin-fixed, paraffin-embedded (FFPE) samples, the most common format for archival tumour tissue in anatomical pathology departments, by conducting for the first time RNA-seq analysis in CUSA aspirates. We compared the molecular diagnosis of somatic mutations used in the clinical routine and the gene expression profiles of fixed solid material from CUSA aspirates and TCRs from the same patients in select

SUBMITTER: Alenda C 

PROVIDER: S-EPMC8297856 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature