In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission.

ABSTRACT: Early events in retrovirus transmission are determined by interactions between incoming viruses and frontline cells near entry sites. Despite their importance for retroviral pathogenesis, very little is known about these events. We developed a bioluminescence imaging (BLI)-guided multiscale imaging approach to study these events in vivo. Engineered murine leukemia reporter viruses allowed us to monitor individual stages of retrovirus life cycle including virus particle flow, virus entry into cells, infection and spread for retroorbital, subcutaneous, and oral routes. BLI permitted temporal tracking of orally administered retroviruses along the gastrointestinal tract as they traversed the lumen through Peyer's patches to reach the draining mesenteric sac. Importantly, capture and acquisitio

SUBMITTER: Haugh KA 

PROVIDER: S-EPMC8298093 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature