Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium.

ABSTRACT: The liver hormone hepcidin regulates systemic iron homeostasis. Hepcidin is also expressed by the kidney, but exclusively in distal nephron segments. Several studies suggest hepcidin protects against kidney damage involving Fe²⁺ overload. The nephrotoxic non-essential metal ion Cd²⁺ can displace Fe²⁺ from cellular biomolecules, causing oxidative stress and cell death. The role of hepcidin in Fe²⁺ and Cd²⁺ toxicity was assessed in mouse renal cortical [mCCD(cl.1)] and inner medullary [mIMCD₃] collecting duct cell lines. Cells were exposed to equipotent Cd²⁺ (0.5-5 μmol/l) and/or Fe²⁺ (50-100 μmol/l) for 4-24 h. Hepcidin (Hamp1) was transiently silenced by RNAi or overexpressed by plasmid transfection. He

SUBMITTER: Probst S 

PROVIDER: S-EPMC8298330 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature