Effects of DNA preservation solution and DNA extraction methods on microbial community profiling of soil.

ABSTRACT: Microbial community profiling using high-throughput sequencing relies in part on the preservation of the DNA and the effectiveness of the DNA extraction method. This study aimed at understanding to what extent these parameters affect the profiling. We obtained samples treated with and without a preservation solution. Also, we compared DNA extraction kits from Qiagen and Zymo-Research. The types of samples were defined strains, both as single species and mixtures, as well as undefined indigenous microbial communities from soil. We show that the use of a preservation solution resulted in substantial changes in the 16S rRNA gene profiles either due to an overrepresentation of Gram-positive bacteria or to an underrepresentation of Gram-negative bacteria. In addition, 16S rRNA gene profiles wer

SUBMITTER: Iturbe-Espinoza P 

PROVIDER: S-EPMC8298342 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature