Rapid detection of phenotypes Bombay se^del and nonsecretor rs200157007 SNP (302C > T) by real-time PCR-based methods.

ABSTRACT: The se^del allele is one of the nonsecretor alleles (se) of FUT2 generated by an Alu-mediated recombination event and was first found in Indian Bombay phenotype individuals who have anti-H, anti-A, and anti-B antibodies in their serum. As well as anti-A, and anti-B antibodies, anti-H is clinically significant because it causes sever hemolytic transfusion reactions. Like se^del, se³⁰² having a missense single nucleotide polymorphism (SNP), 302C > T, is characteristic of South Asians with a frequency of 10-30%. We developed a real-time PCR melting curve analysis for detection of se^del using a 127-bp amplicon encompassing the breakpoint junction. In addition, by performing duplex PCR by amplifying a 65-bp amplicon of the FUT2 coding region at the same

SUBMITTER: Soejima M 

PROVIDER: S-EPMC8298435 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature