Sex-Specific Linear Polyubiquitination Is a Critical Regulator of Contextual Fear Memory Formation.

ABSTRACT: Strong evidence supports that protein ubiquitination is a critical regulator of fear memory formation. However, as this work has focused on protein degradation, it is currently unknown whether polyubiquitin modifications that are independent of the proteasome are involved in learning-dependent synaptic plasticity. Here, we present the first evidence that atypical linear (M1) polyubiquitination, the only ubiquitin chain that does not occur at a lysine site and is largely independent of the proteasome, is critically involved in contextual fear memory formation in the amygdala in a sex-specific manner. Using immunoblot and unbiased proteomic analyses, we found that male (49) and female (14) rats both had increased levels of linear polyubiquitinated substrates following fear conditioning, thou

SUBMITTER: Musaus M 

PROVIDER: S-EPMC8298817 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature