CMIP interacts with WT1 and targets it on the proteasome degradation pathway.

ABSTRACT:

Background

The Wilms tumor 1 suppressor gene, WT1, is expressed throughout life in podocytes and is essential for their function. Downregulation of WT1 has been reported in podocyte diseases but the underlying mechanisms remain unclear. Podocyte injury is the hallmark of idiopathic nephrotic syndrome (INS), the most frequent glomerular disease in children and young adults. An increase in the abundance of Cmaf-inducing protein (CMIP) has been found to alter podocyte function, but it is not known whether CMIP affects WT1 expression.

Methods

Transcriptional and post-transcriptional regulation of WT1in the presence of CMIP was studied using transient transfection, mouse models, and siRNA handling.

Results

We showed that overproduction of CMIP in the podocyte was consisten

SUBMITTER: Zhang SY 

PROVIDER: S-EPMC8299046 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature