Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung.

ABSTRACT:

Background

Cellular diversity of the lung endothelium has not been systematically characterized in humans. We provide a reference atlas of human lung endothelial cells (ECs) to facilitate a better understanding of the phenotypic diversity and composition of cells comprising the lung endothelium.

Methods

We reprocessed human control single-cell RNA sequencing (scRNAseq) data from 6 datasets. EC populations were characterized through iterative clustering with subsequent differential expression analysis. Marker genes were validated by fluorescent microscopy and in situ hybridization. scRNAseq of primary lung ECs cultured in vitro was performed. The signaling network between different lung cell types was studied. For cross-species analysis or disease relevance, we applied the sa

SUBMITTER: Schupp JC 

PROVIDER: S-EPMC8300155 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature