Comparative Proteomic Analysis of Young and Adult Bull (Bos taurus) Cryopreserved Semen.

ABSTRACT: The age of the bull is widely accepted to influence the production of sperm, affecting the amount and quality of produced semen, which in turn impacts the results of cryopreservation. However, the exact influence of the maturation process on cryopreserved sperm, as well as the underlying molecular mechanisms of this process, are not fully understood. The goal of this study was to evaluate changes in the proteome of thawed semen (spermatozoa and supernatant) collected from young and adult bulls (n = 6) using the 2D-DIGE approach. The quality of semen was assessed using a CASA system and flow cytometry. We found no significant age-related variation in semen quality, with the exception of the average path velocity of sperm movement, which was higher in adult bulls. Proteomic analysis indicate

SUBMITTER: Westfalewicz B 

PROVIDER: S-EPMC8300238 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature