Reactive Species from Two-Signal Activated Macrophages Interfere with Their Oxygen Consumption Measurements.

ABSTRACT: Metabolic modulation of macrophage activation has emerged as a promising strategy lately in immunotherapeutics. However, macrophages have a broad spectrum of functions and thus, understanding the exact metabolic changes that drive a particular immune response, is of major importance. In our previous work, we have reported a key role of nitric oxide (NO●) in two(2)-signal activated macrophages [M(2-signals)]. Further characterization using metabolic analysis in intact cells, showed that the basal and maximal respiration levels of M(2-signals) were comparable, with cells being unresponsive to the injections-inducd mitochondrial stress. Here, we show that excessive NO● secretion by the M(2-signals) macrophages, interferes with the oxygen (O2) consumption measurements on cells using the seahor

SUBMITTER: Christopoulos PF 

PROVIDER: S-EPMC8301004 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature