Lipid droplet screen in human hepatocytes identifies TRRAP as a regulator of cellular triglyceride metabolism.
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ABSTRACT:
SUBMITTER: Abbey D
PROVIDER: S-EPMC8301584 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Lipid droplet screen in human hepatocytes identifies TRRAP as a regulator of cellular triglyceride metabolism.
Abbey Deepti D Conlon Donna D Rainville Christopher C Elwyn Susannah S Quiroz-Figueroa Katherine K Billheimer Jeffrey J Schultz David C DC Hand Nicholas J NJ Cherry Sara S Rader Daniel J DJ
Clinical and translational science 20210622 4
Hepatocytes store triglycerides (TGs) in the form of lipid droplets (LDs), which are increased in hepatosteatosis. The regulation of hepatic LDs is poorly understood and new therapies to reduce hepatosteatosis are needed. We performed a siRNA kinase and phosphatase screen in HuH-7 cells using high-content automated imaging of LDs. Changes in accumulated lipids were quantified with developed pipeline that measures intensity, area, and number of LDs. Selected "hits," which reduced lipid accumulati ...[more]
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