Tumor Extracellular Vesicles Regulate Macrophage-Driven Metastasis through CCL5.

ABSTRACT:

Purpose

To understand how tumor cells alter macrophage biology once they are recruited to triple-negative breast cancer (TNBC) tumors by CCL5.

Method

Mouse bone marrow derived macrophage (BMDMs) were isolated and treated with recombinant CCL5 protein alone, with tumor cell conditioned media, or with tumor extracellular vesicles (EVs). Media from these tumor EV-educated macrophages (TEMs) was then used to determine how these macrophages affect TNBC invasion. To understand the mechanism, we assayed the cytokine secretion from these macrophages to determine how they impact tumor cell invasion. Tumor CCL5 expression was varied in tumors to determine its role in regulating macrophage biology through EVs.

Results

Tumor EVs are a necessary component for programming naïve mac

SUBMITTER: Rabe DC 

PROVIDER: S-EPMC8303898 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature