Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester.

ABSTRACT: Conjugation of phytohormones with glucose is a means of modulating their activities, which can be rapidly reversed by the action of β-glucosidases. Evaluation of previously characterized recombinant rice β-glucosidases found that nearly all could hydrolyze abscisic acid glucose ester (ABA-GE). Os4BGlu12 and Os4BGlu13, which are known to act on other phytohormones, had the highest activity. We expressed Os4BGlu12, Os4BGlu13 and other members of a highly similar rice chromosome 4 gene cluster (Os4BGlu9, Os4BGlu10 and Os4BGlu11) in transgenic Arabidopsis. Extracts of transgenic lines expressing each of the five genes had higher β-glucosidase activities on ABA-GE and gibberellin A₄ glucose ester (GA₄-GE). The β-glucosidase expression lin

SUBMITTER: Kongdin M 

PROVIDER: S-EPMC8303963 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature