Engineering Zymomonas mobilis for the Production of Xylonic Acid from Sugarcane Bagasse Hydrolysate.

ABSTRACT: Sugarcane bagasse is an agricultural residue rich in xylose, which may be used as a feedstock for the production of high-value-added chemicals, such as xylonic acid, an organic acid listed as one of the top 30 value-added chemicals on a NREL report. Here, Zymomonas mobilis was engineered for the first time to produce xylonic acid from sugarcane bagasse hydrolysate. Seven coding genes for xylose dehydrogenase (XDH) were tested. The expression of XDH gene from Paraburkholderia xenovorans allowed the highest production of xylonic acid (26.17 ± 0.58 g L^-1) from 50 g L^-1 xylose in shake flasks, with a productivity of 1.85 ± 0.06 g L^-1 h^-1 and a yield of 1.04 ± 0.04 g_AX/g_X. Deletion of the xylose reductase gene furth

SUBMITTER: Herrera CRJ 

PROVIDER: S-EPMC8304316 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature