An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine.

ABSTRACT: Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm's intestine is therefore of great interest, in particular with respect to normal bacterial digestion or intestinal colonization by pathogens. Here, we report an in vivo study of bacteria in the gut of C. elegans. We take advantage of a polydimethylsiloxane (PDMS) microfluidic device enabling passive immobilization of adult worms under physiological conditions. Non-pathogenic Escherichia coli (E. coli) bacteria expressing either pH-sensitive or pH-insensitive fluorescence reporters as well as fluorescently marked indigestible microbeads w

SUBMITTER: Viri V 

PROVIDER: S-EPMC8304684 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature