Cloning and Expression Analysis of Flavonoid 3', 5'-Hydroxylase Gene from Brunfelsia acuminata.
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ABSTRACT:
SUBMITTER: Li M
PROVIDER: S-EPMC8304711 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
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Cloning and Expression Analysis of Flavonoid 3', 5'-Hydroxylase Gene from <i>Brunfelsia acuminata</i>.
Li Min M Cao Yuting Y Debnath Biswojit B Yang Hongjuan H Kui Xiaohua X Qiu Dongliang D
Genes 20210718 7
The full-length cDNA sequence of <i>F3'5'H</i> gene from the <i>Brunfelsia acuminata</i> was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5'UTR and 61 bp 3'UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3'5'H was 91%, 87% and 84% with that of <i>Petunia × hy ...[more]
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