Cysteine Proteome Reveals Response to Endogenous Oxidative Stress in Bacillus cereus.

ABSTRACT: At the end of exponential growth, aerobic bacteria have to cope with the accumulation of endogenous reactive oxygen species (ROS). One of the main targets of these ROS is cysteine residues in proteins. This study uses liquid chromatography coupled to high-resolution tandem mass spectrometry to detect significant changes in protein abundance and thiol status for cysteine-containing proteins from Bacillus cereus during aerobic exponential growth. The proteomic profiles of cultures at early-, middle-, and late-exponential growth phases reveals that (i) enrichment in proteins dedicated to fighting ROS as growth progressed, (ii) a decrease in both overall proteome cysteine content and thiol proteome redox status, and (iii) changes to the reduced thiol status of some key proteins, such as

SUBMITTER: Hamitouche F 

PROVIDER: S-EPMC8305198 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature