Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD.

ABSTRACT:

Background

Beta cell identity changes occur in the islets of donors with diabetes, but the molecular basis of this remains unclear. Protecting residual functional beta cells from cell identity changes may be beneficial for patients with diabetes.

Results

A somatostatin-positive cell population was induced in stressed clonal human EndoC-βH1 beta cells and was isolated using FACS. A transcriptomic characterisation of somatostatin-positive cells was then carried out. Gain of somatostatin-positivity was associated with marked dysregulation of the non-coding genome. Very few coding genes were differentially expressed. Potential candidate effector genes were assessed by targeted gene knockdown. Targeted knockdown of the HNRNPD gene induced the emergence of a somatostatin-positive

SUBMITTER: Jeffery N 

PROVIDER: S-EPMC8305497 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature