Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs.

ABSTRACT: Metabolomics is a technique that allows for the evaluation of the entire extractable chemical profile of a plant, for example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due to drought. Metabolomic analysis is dependent upon the efficiency of the extraction protocol. Currently, there are two common extraction procedures widely used in metabolomic experiments, those that extract from plant tissue processed in liquid nitrogen or extraction from lyophilised plant tissues. Here, we evaluated the two using non-targeted metabolomics to show that lyophilisation can stabilise the maize (Zea mays) extractable metabolome, increasing throughput and efficiency of extraction as compared to the more traditional processing in liquid ni

SUBMITTER: Gaffney I 

PROVIDER: S-EPMC8305929 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature