Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis.

ABSTRACT:

Background

The ability to achieve high peak viable cell density earlier in CHO cell culture and maintain an extended cell viability throughout the production process is highly desirable to increase recombinant protein yields, reduce host cell impurities for downstream processing and reduce the cost of goods. In this study we implemented label-free LC-MS/MS proteomic profiling of IgG4 producing CHO cell lines throughout the duration of the cell culture to identify differentially expressed (DE) proteins and intracellular pathways associated with the high peak viable cell density (VCD) and extended culture VCD phenotypes.

Results

We identified key pathways in DNA replication, mitotic cell cycle and evasion of p53 mediated apoptosis in high peak VCD clonally derived cell lines (

SUBMITTER: Bryan L 

PROVIDER: S-EPMC8305936 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature