Genome Characterization of Nocturne116, Novel Lactococcus lactis-Infecting Phage Isolated from Moth.

ABSTRACT: While looking for novel insect-associated phages, a unique siphophage, Nocturne116, was isolated from a deceased local moth specimen along with its host, which was identified by 16S rRNA gene sequencing as a strain of Lactococcus lactis. Next-generation sequencing and the subsequent genome annotation elaborated on herein revealed that the genome of Nocturne116 is a 25,554 bp long dsDNA molecule with 10 bp long 3' cos overhangs and a GC content of 37.99%, comprising 52 predicted open reading frames. The complete nucleotide sequence of phage Nocturne116 genome is dissimilar to any of the already sequenced phages, save for a distant link with Lactococcus phage Q54. Functions for only 15/52 of Nocturne116 gene products could be reliably predicted using contemporary comparative ge

SUBMITTER: Zrelovs N 

PROVIDER: S-EPMC8306868 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature