Influence of Subcellular Localization and Functional State on Protein Turnover.

ABSTRACT: Protein homeostasis is an equilibrium of paramount importance that maintains cellular performance by preserving an efficient proteome. This equilibrium avoids the accumulation of potentially toxic proteins, which could lead to cellular stress and death. While the regulators of proteostasis are the machineries controlling protein production, folding and degradation, several other factors can influence this process. Here, we have considered two factors influencing protein turnover: the subcellular localization of a protein and its functional state. For this purpose, we used an imaging approach based on the pulse-labeling of 17 representative SNAP-tag constructs for measuring protein lifetimes. With this approach, we obtained precise measurements of protein turnover rates in several subcellul

SUBMITTER: Yousefi R 

PROVIDER: S-EPMC8306977 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature