CRISPR loci-PCR as Tool for Tracking Azospirillum sp. Strain B510.

ABSTRACT: Azospirillum-based plant and soil inoculants are widely used in agriculture. The inoculated Azospirillum strains are commonly tracked by both culture-dependent and culture-independent methods, which are time-consuming or expensive. In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species. Here, we investigated the use of CRISPR loci to track specific Azospirillum strains in soils systems by PCR. Primer sets for Azospirillum sp. strain B510 were designed and evaluated by colony and endpoint PCR. The CRISPR_loci-PCR approach was standardized for Azospirillum sp. strain B510, and its specificity was observed by testing against 9 d

SUBMITTER: Rilling JI 

PROVIDER: S-EPMC8307223 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature