Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.

ABSTRACT: Chimeric terpene synthases, which consist of C-terminal prenyltransferase (PT) and N-terminal class I terpene synthase (TS) domains (termed PTTSs here), is unique to fungi and produces structurally diverse di- and sesterterpenes. Prior to this study, 20 PTTSs had been functionally characterized. Our understanding of the origin and functional evolution of PTTS genes is limited. Our systematic search of sequenced fungal genomes among diverse taxa revealed that PTTS genes were restricted to Dikarya. Phylogenetic findings indicated different potential models of the origin and evolution of PTTS genes. One was that PTTS genes originated in the common Dikarya ancestor and then underwent frequent gene loss among various subsequent lineages. To understand their functiona

SUBMITTER: Chen R 

PROVIDER: S-EPMC8307374 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature