Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation.

ABSTRACT: During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared

SUBMITTER: Kupper N 

PROVIDER: S-EPMC8308011 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature