Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri.

ABSTRACT: Aliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments. Whether natural transformation can be a mechanism that generates genetic diversity in A. butzleri is still unknown. In the present study, we aimed to establish if A. butzleri is naturally competent for transformation and to investigate the factors influencing this process. Two different transformation procedures were tested using exogenous and isogenic DNA containing antibiotic resistance markers, and different external conditions influencing the process were evaluated. The highest number of transformable A. butzleri strains were obtained with the agar transformation method when compared to the biphasic system (65% versus 47%). A

SUBMITTER: Bonifacio M 

PROVIDER: S-EPMC8308473 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature