High-Throughput Phenotypic Assay to Screen for Anthelmintic Activity on Haemonchus contortus.

ABSTRACT: Parasitic worms cause very significant diseases in animals and humans worldwide, and their control is critical to enhance health, well-being and productivity. Due to widespread drug resistance in many parasitic worms of animals globally, there is a major, continuing demand for the discovery and development of anthelmintic drugs for use to control these worms. Here, we established a practical, cost-effective and semi-automated high throughput screening (HTS) assay, which relies on the measurement of motility of larvae of the barber's pole worm (Haemonchus contortus) using infrared light-interference. Using this assay, we screened 80,500 small molecules and achieved a hit rate of 0.05%. We identified three small molecules that reproducibly inhibited larval motility and/or development (IC50 v

SUBMITTER: Taki AC 

PROVIDER: S-EPMC8308562 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature