Evidence of Immune Modulators in the Secretome of the Equine Tapeworm Anoplocephala perfoliata.

ABSTRACT: Anoplocephala perfoliata is a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of A. perfoliata is hampered by a lack of tools to better understand the host-parasite interface. This interface is likely influenced by parasite derived immune modulators released in the secretome as free proteins or components of extracellular vesicles (EVs). Therefore, adult RNA was sequenced and de novo assembled to generate the first A. perfoliata transcriptome. In addition, excretory secretory products (ESP) from adult A. perfoliata were collected and EVs isolated using size exclusion chromatography, prior to proteomic analysis of the EVs, the EV surface and EV depleted ESP. Transcriptome analysis revealed 454 sequences homologou

SUBMITTER: Wititkornkul B 

PROVIDER: S-EPMC8308605 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature