Dataset Information

The First Detection and Genetic Characterization of Four Different Honeybee Viruses in Wild Bumblebees from Croatia.

ABSTRACT: To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus-ABPV, black queen cell virus-BQCV, chronic bee paralysis virus-CBPV, deformed wing virus-DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified positive viruses were in the following decreasing order: BQCV > DWV > ABPV, CBPV. Additionally, direct sequencing of samples positive for BQCV (n = 24) and DWV (n = 2) was performed, as well as a test of molecular phylogeny comparison with those available in GenBank. Selected positive field viruses' strains showed 95.7 to 100% (BQCV) and 98.09% (DWV) nucleotide identity with previously detected and deposited honeybee virus strains in the geographic areas in Croatia and neighboring Slovenia. In this article, the first detection of four honeybee viruses with genetic characterization of high diversity strains circulating in wild bumblebees in Croatia is presented.

SUBMITTER: Tlak Gajger I

PROVIDER: S-EPMC8308666 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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The First Detection and Genetic Characterization of Four Different Honeybee Viruses in Wild Bumblebees from Croatia.

Tlak Gajger Ivana I Šimenc Laura L Toplak Ivan I

Pathogens (Basel, Switzerland) 20210625 7

To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus-ABPV, black queen cell virus-BQCV, chronic bee paralysis virus-CBPV, deformed wing virus-DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified ...[more]

PMID: 34202101

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Project description:Field-grown wheat (Triticum aestivum L.) plants can be co-infected by multiple viruses, including wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), brome mosaic virus (BMV), and barley stripe mosaic virus (BSMV). These viruses belong to four different genera in three different families and are, hence, genetically divergent. However, the impact of potential co-infections with two, three, or all four of them on the viruses themselves, as well as the wheat host, has yet to be examined. This study examined bi-, tri-, and quadripartite interactions among these viruses in wheat for disease development and accumulation of viral genomic RNAs, in comparison with single virus infections. Co-infection of wheat by BMV and BSMV resulted in BMV-like symptoms with a drastic reduction in BSMV genomic RNA copies and coat protein accumulation, suggesting an antagonism-like effect exerted by BMV toward BSMV. However, co-infection of either BMV or BSMV with WSMV or TriMV led to more severe disease than singly infected wheat, but with a decrease or no significant change in titers of interacting viruses in the presence of BMV or BSMV, respectively. These results were in stark contrast with exacerbated disease phenotype accompanied with enhanced virus titers caused by WSMV and TriMV co-infection. Co-infection of wheat by WSMV, TriMV, and BMV or BSMV resulted in enhanced synergistic disease accompanied by increased accumulation of TriMV and BMV but not WSMV or BSMV. Quadripartite interactions in co-infected wheat by all four viruses resulted in very severe disease synergism, leading to the death of the most infected plants, but paradoxically, a drastic reduction in BSMV titer. Our results indicate that interactions among different viruses infecting the same plant host are more complex than previously thought, do not always entail increases in virus titers, and likely involve multiple mechanisms. These findings lay the foundation for additional mechanistic dissections of synergistic interactions among unrelated plant viruses.

Dataset Information

The First Detection and Genetic Characterization of Four Different Honeybee Viruses in Wild Bumblebees from Croatia.

Publications

The First Detection and Genetic Characterization of Four Different Honeybee Viruses in Wild Bumblebees from Croatia.

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