Spatiotemporal and Quantitative Monitoring of the Fate of "Candidatus Phytoplasma Solani" in Tomato Plants Infected by Grafting.

ABSTRACT: Understanding how phytoplasmas move and multiply within the host plant is fundamental for plant-pathogen interaction studies. In recent years, the tomato has been used as a model plant to study this type of interaction. In the present work, we investigated the distribution and multiplication dynamics of one strain of "Candidatus Phytoplasma (Ca. P.) solani", (16SrXII-A) in tomato (Solanum lycopersicum L., cv. Micro-Tom) plants. We obtained infected plants by grafting, a fast and effective method to maintain phytoplasma infection. In planta spread and multiplication of "Ca. P. solani" was monitored over time using qualitative and quantitative qPCR. Root, apical shoot, lower leaves, and upper leaves were sampled at each sampling time. We hypothesized that "Ca. P. solani" from the grafting si

SUBMITTER: Carminati G 

PROVIDER: S-EPMC8308695 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature