Comparison of Serological and Molecular Assays for Bartonella Species in Dogs with Hemangiosarcoma.

ABSTRACT: Currently, a gold standard diagnostic test for Bartonella infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported Bartonella spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital P

SUBMITTER: Lashnits E 

PROVIDER: S-EPMC8308881 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature