The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus).
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ABSTRACT: The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detec
SUBMITTER: Karamon J
PROVIDER: S-EPMC8308913 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
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