Simplified ⁸⁹Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells.

ABSTRACT: In this work, a method for the preparation of the highly lipophilic labeling synthon [⁸⁹Zr]Zr(oxinate)₄ was optimized for the radiolabeling of liposomes and human induced pluripotent stem cells (hiPSCs). The aim was to establish a robust and reliable labeling protocol for enabling up to one week positron emission tomography (PET) tracing of lipid-based nanomedicines and transplanted or injected cells, respectively. [⁸⁹Zr]Zr(oxinate)₄ was prepared from oxine (8-hydroxyquinoline) and [⁸⁹Zr]Zr(OH)₂(C₂O₄). Earlier introduced liquid-liquid extraction methods were simplified by the optimization of buffering, pH, temperature and reaction times. For quality control, thin-layer chromatography (TLC), size-exclusi

SUBMITTER: Polyak A 

PROVIDER: S-EPMC8309181 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature