Cell Viability Assays in Three-Dimensional Hydrogels: A Comparative Study of Accuracy.

ABSTRACT: Three-dimensional (3D) cell culture systems, such as tumor organoids and multicellular tumor spheroids, have been developed in part as a result of major advances in tissue engineering and biofabrication techniques. 3D cell culture offers great capabilities in drug development, screening, testing, and precision medicine owing to its physiological accuracy. However, since the inception of 3D systems, few methods have been reported to successfully analyze cell viability quantitatively within hydrogel constructs. In this study, we describe and compare commercially available viability assays developed for two-dimensional (2D) applications for use in 3D constructs composed of organic, synthetic, or hybrid hydrogel formulations. We utilized Promega's CellTiter-Glo^®, CellTiter-Glo 3D, a

SUBMITTER: Dominijanni AJ 

PROVIDER: S-EPMC8309413 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature